| Rank | Change from 2008[1] | School district | State/Territory | Students in Fall 2010[2] |
| 1 | 0 | New York City Department of Education | New York | 995,336 |
| 2 | 0 | Los Angeles Unified School District | California | 667,273 |
| 3 | 0 | Departamento de Educación de Puerto Rico | Puerto Rico | 437,202[3] |
| 4 | 0 | Chicago Public Schools | Illinois | 405,644 |
| 5 | +1 | Miami-Dade County Public Schools | Florida | 347,366 |
| 6 | -1 | Clark County School District | Nevada | 314,059 |
| 7 | 0 | Broward County Public Schools | Florida | 256,472 |
| 8 | 0 | Houston Independent School District | Texas | 204,245 |
| 9 | 0 | Hillsborough County Public Schools | Florida | 194,525 |
| 10 | 0 | Hawaiʻi Department of Education | Hawaii | 179,601 |
| 11 | +2 | Orange County Public Schools | Florida | 176,008 |
| 12 | +2 | School District of Palm Beach County | Florida | 174,663 |
| 13 | -1 | Fairfax County Public Schools | Virginia | 174,479 |
| 14 | -3 | School District of Philadelphia | Pennsylvania | 166,233 |
| 15 | +2 | Gwinnett County Public Schools | Georgia | 160,744 |
| 16 | -1 | Dallas Independent School District | Texas | 157,162 |
| 17 | +5 | Wake County Public School System | North Carolina | 144,173 |
| 18 | +1 | Montgomery County Public Schools | Maryland | 144,023 |
| 19 | +2 | Charlotte-Mecklenburg Schools | North Carolina | 135,954 |
| 20 | 0 | San Diego Unified School District | California | 131,785 |
| 21 | -2 | Prince George's County Public Schools | Maryland | 126,671 |
| 22 | -6 | Duval County Public Schools | Florida | 123,997 |
| 23 | 0 | Memphis City Schools | Tennessee | 111,834 |
| 24 | +2 | Cobb County School District | Georgia | 107,315 |
| 25 | +7 | Cypress-Fairbanks Independent School District | Texas | 106,097 |
| 26 | +8 | Baltimore County Public Schools | Maryland | 104,160 |
| 27 | -2 | Pinellas County Schools | Florida | 104,001 |
| 28 | 0 | DeKalb County School System | Georgia | 98,115 |
| 29 | +2 | Jefferson County Public Schools | Kentucky | 97,331 |
| 30 | +8 | Northside Independent School District | Texas | 95,581 |
| 31 | -2 | Albuquerque Public Schools | New Mexico | 95,415 |
| 32 | -2 | Polk County Public Schools | Florida | 95,178 |
| 33 | +4 | Fulton County School System | Georgia | 91,864 |
| 34 | +1 | Jefferson County Public Schools | Colorado | 85,979 |
| 35 | +4 | Austin Independent School District | Texas | 85,697 |
| 36 | -3 | Long Beach Unified School District | California | 84,812 |
| 37 | -1 | Baltimore City Public School System | Maryland | 83,800 |
| 38 | +3 | School District of Lee County | Florida | 81,967 |
| 39 | +1 | Fort Worth Independent School District | Texas | 81,651 |
| 40 | -6 | Milwaukee Public Schools | Wisconsin | 80,934 |
| 41 | +11 | Prince William County Public Schools | Virginia | 79,358 |
| 42 | +4 | Metropolitan Nashville Public Schools | Tennessee | 78,782 |
| 43 | +5 | Denver Public Schools | Colorado | 78,339 |
| 44 | -21 | Detroit Public Schools | Michigan | 77,757 |
| 45 | +2 | Anne Arundel County Public Schools | Maryland | 75,481 |
| 46 | -3 | Fresno Unified School District | California | 74,833 |
| 47 | +4 | Guilford County Schools | North Carolina | 73,205 |
| 48 | +2 | Greenville County School District | South Carolina | 71,930 |
| 49 | -5 | Brevard Public Schools | Florida | 71,866 |
| 50 | -1 | Virginia Beach City Public Schools | Virginia | 71,185 |
| 51 | +2 | Granite School District | Utah | 70,083 |
| 52 | +2 | Fort Bend Independent School District | Texas | 68,948 |
| 53 | +9 | Davis School District | Utah | 67,452 |
| 54 | +19 | Alpine School District | Utah | 67,076 |
| 55 | +4 | Pasco County Schools | Florida | 66,994 |
| 56 | +8 | North East Independent School District | Texas | 66,604 |
| 57 | -12 | Mesa Public Schools | Arizona | 65,123 |
| 58 | +2 | Arlington Independent School District | Texas | 64,484 |
| 59 | -1 | Washoe County School District | Nevada | 64,380 |
| 60 | +1 | El Paso Independent School District | Texas | 64,330 |
| 61 | -6 | Seminole County Public Schools | Florida | 64,229 |
| 62 | +5 | Aldine Independent School District | Texas | 63,154 |
| 63 | +24 | Loudoun County Public Schools | Virginia | 63,142 |
| 64 | -1 | Elk Grove Unified School District | California | 62,455 |
| 65 | -8 | Mobile County Public School System | Alabama | 62,016 |
| 66 | -10 | Volusia County Schools | Florida | 61,559 |
| 67 | +20 | Douglas County School District RE-1 | Colorado | 61,465 |
| 68 | +17 | Katy Independent School District | Texas | 60,803 |
| 69 | -1 | Chesterfield County Public Schools | Virginia | 59,243 |
| 70 | -4 | Knox County Schools | Tennessee | 57,977 |
| 71 | 0 | Garland Independent School District | Texas | 57,833 |
| 72 | -2 | Santa Ana Unified School District | California | 57,319 |
| 73 | +1 | Boston Public Schools | Massachusetts | 56,037 |
| 74 | +4 | San Francisco Unified School District | California | 55,571 |
| 75 | +5 | Plano Independent School District | Texas | 55,568 |
| 76 | +1 | San Antonio Independent School District | Texas | 55,116 |
| 77 | -8 | San Bernardino City Unified School District | California | 54,518 |
| 78 | +6 | Winston-Salem/Forsyth County Schools | North Carolina | 53,367 |
| 79 | +3 | School District of Osceola County, Florida | Florida | 53,357 |
| 80 | -1 | Cumberland County Schools | North Carolina | 53,307 |
| 81 | -16 | Tucson Unified School District | Arizona | 53,275 |
| 82 | +1 | Capistrano Unified School District | California | 53,192 |
| 83 | +6 | Corona-Norco Unified School District | California | 53,149 |
| 84 | +7 | Cherry Creek School District | Colorado | 52,232 |
| 85 | +5 | Pasadena Independent School District | Texas | 52,218 |
| 86 | +9 | Lewisville Independent School District | Texas | 51,484 |
| 87 | new | Conroe Independent School District | Texas | 51,170 |
| 88 | -13 | Columbus City Schools | Ohio | 51,134 |
| 89 | +7 | Howard County Public Schools | Maryland | 50,994 |
| 90 | -9 | Clayton County Public Schools | Georgia | 50,366 |
| 91 | -49[4] | Jordan School District | Utah | 50,048 |
| 92 | +6 | Brownsville Independent School District | Texas | 49,879 |
| 93 | -7 | Atlanta Public Schools | Georgia | 54,956 |
| 94 | new | Omaha Public Schools | Nebraska | 49,405 |
| 95 | +5 | Henrico County Public Schools | Virginia | 49,405 |
| 96 | new | Wichita Public Schools | Kansas | 49,329 |
| 97 | -4 | Anchorage School District | Alaska | 49,206 |
| 98 | -1 | Garden Grove Unified School District | California | 48,659 |
| 99 | -7 | Sacramento City Unified School District | California | 47,897 |
| 100 | new | Seattle Public Schools | Washington | 47,735 |
| 101 | 0 | Shelby County Schools | Tennessee | 47,706 |